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. 2011 Aug 23;62(15):5419–5428. doi: 10.1093/jxb/err212

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — Single-molecule fluorescence microscopy in root cells. Single-molecule analysis of EGFP in root cells using the microtubule marker (MAP4–GFP). (a) The microtubule marker (MAP4–GFP) imaged under TIRFM, VAEM, and epifluorescence illumination. Scale bars=10 μm. (b) The same field of view as (a), after 75 min bleaching under epi-illumination. (c) Total intensity of a single MAP4–GFP marker through time, showing the typical intensity and blinking of a single GFP molecule. (d) Detail of one frame from the time series. The box indicates the single molecule. The molecule analysed here was located as indicated by the arrow in 6a and b. Scale bars=1 μm. (e) As (d), but with the path of the molecule superimposed. The molecule’s initial position was at the top left end. (f) Total intensity and position of the fluorescent spot through time.