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BTPC of COS integument is phosphorylated at Ser-425. Clarified integument extracts were incubated in the presence (+) and absence (–) of λ-phosphatase prior to SDS-PAGE and immunoblotting with anti-BTPC or anti-pSer425 with 10 μg ml−1 of the corresponding dephospho- or phospho-blocking peptide (50 μg protein per lane).
