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. 2011 Aug 23;62(15):5671–5682. doi: 10.1093/jxb/err253

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 3. — Immunogold localization of VpRFP1 in Vitis pseudoreticulata leaves. (A) With use of the rabbit pre-immune serum instead of the specific rabbit antiserum as control, no substantial signal was detected. (B) The absence of the antiserum to test the non-specific labelling of goat anti-rabbit IgG antibody–gold conjugate; no substantial signal was detected. (C) Before inoculation with pathogen, a few gold particles were detected in the nucleus and cytoplasm. (D) At 24 h after inoculation with pathogen, more gold particles were detected in the nucleus. N, nucleus; Nu, nucleolus; Cy, cytoplasm; CW, cell wall. (This figure is available in colour at JXB online.)