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Soybean arginase expression in developing embryos and in germinating seeds. A, Autoradiogram of blot containing total RNA isolated from developing embryos (30 μg) and whole seedlings (8 μg). An embryo water content of 8% corresponds to quiescent seed. Hybridization was carried out using radiolabeled AG1 cDNA as a probe. B, Uniformity of RNA loading and transfer was confirmed by nylon membrane staining with methylene blue. Numbers at left indicate marker sizes.
