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. 2011 Nov 23;6(11):e27940. doi: 10.1371/journal.pone.0027940

Figure 2. Structure of exon 5, intron 5, and exon 6 of canine LHX3.

Figure 2

(A) The DNA sequence derived from BAC clone RP81-17A4 fills a gap in the reference genome sequence build 2.1. The intron is indicated by bold characters and the putative splice branch site is underlined. The intron contained 6 repeats of the 7 bp sequence GCGCCCC, indicated by alternating grey and black blocks. The G at position 3 of the third repeat was mutated to a C and the last repeat ends with a G-residue. The Genbank accession number of the DNA sequence is Q913875. (B) Comparison of the DNA sequence of intron 5 in the normal German shepherd dogs B4 and C8 and the dwarfs B3 and C6 after bisulfite treatment of genomic DNA. The treatment converts unmethylated cytosine residues to uracil residues, which act like thymine in the PCR. The intron in the normal dogs B4 and C8 (Fig. S2) consisted of 75 bp and contained 6 repeats of the 7 bp sequence which are converted to GTGTTTT, indicated by alternating grey and black blocks. Seven consecutive nucleotides in the region of repeats 4–6 are deleted from the intron of the dwarfs B3 and C6, indicated by dashes (NM_001197187, c.622-37-31del).