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. 2011 Nov 23;6(11):e27869. doi: 10.1371/journal.pone.0027869

Figure 1. A GA-rich sequence is located near the 5′ region of intron 10 of INSR.

Figure 1

A schematic diagram of minigenes hIR and hIRΔ1.9 is shown. White boxes represent exon 10 and 12 and the black box represents exon 11. The intervening lines indicate the introns in between. Dotted lines indicate deletions. Solid perpendicular lines in minigene hIR and hIRΔ1.9 demonstrate the boundary of the GA rich sequence. Sequences of GA rich region and the series of linker scanning mutants (GA1-10) are shown below. The ratio of isoform IR-B that includes exon 11 to isoform IR-A that excludes exon 11 (± standard deviation) is given at the right hand site for minigene hIRΔ1.9 and hIR as well as the mutants, after transient transfection into HepG2 cells. Asterisks indicate statistical significance (*p≤0.05, ** p≤0.01) vs. wild-type sequence for n = 4 experiments. ND represents not determined. IE1, IE2 and IE3 indicate the intron elements used for subsequent in vitro binding experiments.