Figure 3. Nuclear Run-on analysis depicting the transcriptional activity of the rpL32 genes in shoots.

(A) Pattern of loading of different genes in the dot blots. The blots were hybridized with 3.45×10⁶ cpm/ml probe count. (B) Blot for control (no stress) condition. (C) Blot for salt stress (200 mM NaCl, 24 h) condition. (D) Graphical representation of the results of nuclear run-on assay. Densitometric quantification values for rpL32 genes were normalized with the densitometric readings obtained for 18S rRNA.