Figure 5. Cytoskeletal defects of src64^D404N/src64^KO trans-heterozygotes.

(A) src64^D404N/+, src64^D404N and src64^D404N/src64^KO egg chambers stained with antibody to HTS. Ring canal diameters are not reduced in src64^D404N/+ egg chambers but are reduced in src64^D404N/src64^KO egg chambers. Inset: ring canal of approximately mean diameter, reoriented so that diameter is projected along the vertical axis. Scale bar, 50 µm. (B) Early cellularization stage embryos laid by src64^KO, src64^D404N and src64^D404N/src64^KO mothers. Embryos show non-uniform cellularization fronts. Scale bar, 100 µm. (C) Grazing section projections of early cellularization stage embryos laid by src64^KO, src64^D404N and src64^D404N/src64^KO mothers showing microfilament networks. Microfilament ring defects of maternally trans-heterozygous src64^D404N/src64^KO embryos are similar to those of src64^KO and src64^D404N embryos. Embryos are stained with antibody to myosin II heavy chain. Scale bar, 10 µm. (D) Ring canal diameters. src64^D404N and src64^D404N/src64^KO do not differ (p = 0.35). Error bars are SEM. (E) Microfilament ring circularity during early cellularization. src64^D404N and src64^D404N/src64^KO do not differ (p = 0.52). Error bars are SEM.