Table 1. src64 mutations and fertility of src64^KO trans-heterozygous females.

Allele	Mutationa	Substitutionb	Domain	Eggs laid/female	Eggs hatched (%)
+	-	-	-	498 (n = 4)	84% (n = 1992)
src64Δ17	5′ deletion [12]	-	-	120 (n = 9)	43% (n = 1017)
src64KO	Knockout [14]	-	-	13 (n = 17)	15% (n = 208)
src64P190L	CCT→CTT	P190L	SH2	216 (n = 9)	86% (n = 1853)
src64D204V	GAT→GTT	D204V	SH2	379 (n = 5)	74% (n = 1812)
src64G208E	GGA→GAA	G208E	SH2	390 (n = 5)	84% (n = 1905)
src64R217C	CGT→TGT	R217C	SH2	411 (n = 8)	87% (n = 2794)
src64C259Y	TGC→TAC	C259Y	SH2	322 (n = 8)	67% (n = 2124)
src64D372N	GAT→AAT	D372N	kinase	428 (n = 5)	87% (n = 2138)
src64H402L	CAT→CTT	H402L	kinase	323 (n = 6)	73% (n = 1796)
src64R403C	CGC→TGC	R403C	kinase	332 (n = 5)	88% (n = 1659)
src64D404N	GAT→AAT	D404N	kinase	93 (n = 10)	28% (n = 850)
src64S440F	TCC→TTC	S440F	kinase	412 (n = 9)	96% (n = 2042)

Mutation is shown for each allele. + indicates the wild-type allele. For point mutations, the base pair change, the amino acid substitution and the protein domain containing the substitution are shown. Female fertility was assayed in females trans-heterozygous for the mutation and the src64^KO allele. Number of eggs laid by females over days 3–10 after introduction of males, is shown.

^aAltered nucleotide is underlined.

^bWild-type amino acid is indicated, followed by position and mutant amino acid.