Table 1. src64 mutations and fertility of src64KO trans-heterozygous females.
Allele | Mutationa | Substitutionb | Domain | Eggs laid/female | Eggs hatched (%) |
+ | - | - | - | 498 (n = 4) | 84% (n = 1992) |
src64Δ17 | 5′ deletion [12] | - | - | 120 (n = 9) | 43% (n = 1017) |
src64KO | Knockout [14] | - | - | 13 (n = 17) | 15% (n = 208) |
src64P190L | CCT→CTT | P190L | SH2 | 216 (n = 9) | 86% (n = 1853) |
src64D204V | GAT→GTT | D204V | SH2 | 379 (n = 5) | 74% (n = 1812) |
src64G208E | GGA→GAA | G208E | SH2 | 390 (n = 5) | 84% (n = 1905) |
src64R217C | CGT→TGT | R217C | SH2 | 411 (n = 8) | 87% (n = 2794) |
src64C259Y | TGC→TAC | C259Y | SH2 | 322 (n = 8) | 67% (n = 2124) |
src64D372N | GAT→AAT | D372N | kinase | 428 (n = 5) | 87% (n = 2138) |
src64H402L | CAT→CTT | H402L | kinase | 323 (n = 6) | 73% (n = 1796) |
src64R403C | CGC→TGC | R403C | kinase | 332 (n = 5) | 88% (n = 1659) |
src64D404N | GAT→AAT | D404N | kinase | 93 (n = 10) | 28% (n = 850) |
src64S440F | TCC→TTC | S440F | kinase | 412 (n = 9) | 96% (n = 2042) |
Mutation is shown for each allele. + indicates the wild-type allele. For point mutations, the base pair change, the amino acid substitution and the protein domain containing the substitution are shown. Female fertility was assayed in females trans-heterozygous for the mutation and the src64KO allele. Number of eggs laid by females over days 3–10 after introduction of males, is shown.
Altered nucleotide is underlined.
Wild-type amino acid is indicated, followed by position and mutant amino acid.