Table 6. Genotyping reproducibility of replicate PCR products generated from independent RT-PCR amplification process by 3 different operators in a 5-member proficiency testing panel received from VQA.
| No. of drug resistance mutations | ||||||||
| Sample ID | HIV-1 VL (log10) | HIV-1 Subtype | % Nucleotide sequence identity | Replicate Tests | No. Partially discordant mutation | |||
| IH1* | IH2 | IH3 | TG# | |||||
| 1 | 3.76 | B | 98.83±0.18 | 1 | 1 | 1 | 1 | 0 |
| 2 | 4.13 | C | 99.65±0.23 | 10 | 10 | 7 | 10 | 3 |
| 3 | 4.19 | F | 98.22±0.30 | 0 | 0 | 0 | 0 | 0 |
| 4 | 3.93 | B | 99.08±0.11 | 5 | 5 | 5 | 5 | 0 |
| 5 | 4.75 | C | 99.58±0.08 | 6 | 6 | 5 | 4 | 3 |
*IH1-3: tests were independently performed by 3 operators using the optimized in-house assay; #:TRUGENE® assay.