FIG. 5.

MEHP-suppressed TIMP2 expression is FSH-dependent in vitro. Nuclear extracts (A) and total protein (B) from primary Sertoli cell–germ cell cocultures treated with FSH in the absence or presence of MEHP (200 μM) for 12 h were analyzed by Western blot analysis using antibodies against TIMP2, FSHR, MYC, CREB, and CEBPA. Tubulin and TBP serve as loading controls. C) The amount of soluble TIMP2 from primary coculture cells following MEHP, FSH (50 ng/ml) treatments, and FSH-MEHP cotreatment was measured by ELISA. Values represent means ± SEM. Significant differences between groups are denoted by bars with different letters (*P < 0.05, ANOVA).