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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Mech Dev. 2011 Aug 6;128(7-10):376–386. doi: 10.1016/j.mod.2011.07.004

Fig. 4. Diversin is required for ciliary function in multi-ciliated cells.

Fig. 4

(A, B) Effects of Diversin MO (DivMO) on ciliogenesis. Morpholinos (20 ng each) and RNAs for Centrin2-RFP (0.4 ng) and Mig12-GFP (0.1 ng) were injected as described in Fig. 1, embryos were cultured until stage 34, fixed and stained for cilia with anti-acetylated tubulin antibody. (A) CoMO-injected cell. (B) DivMO-injected cell. Short cilia are marked by arrowheads at the base (red) and the tip (blue). (C) Basal body density is slightly reduced in multi-ciliated cells from embryos injected with DivMO. Number of basal bodies was counted in eight randomly picked multi-ciliated cells of CoMO or DivMO injected embryos. (D) DivMO-injected embryos contain shorter cilia. Cilia length was measured in multi-ciliated cells of CoMO- or DivMO-injected embryos shown in A, B. (E) Effects of DivMO on hovering movements. Morpholinos (20 ng) were injected into four ventral blastomeres of eight-cell embryos. Compared to CoMO, DivMO significantly decreased hovering movement velocity that was measured when control siblings reached stage 36 (see Materials and Methods). Means +/− standard deviations are shown, *p < 0.005.