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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Aug 16;1814(12):1997–2002. doi: 10.1016/j.bbapap.2011.08.004

Figure 3.

Figure 3

Laser flash-induced difference spectrum (550-600 nm) of the partially reduced eNOS holoenzyme (with added CaM) obtained 980 ms after the 450 nm laser pulse. Anaerobic solutions contained 14 μM eNOS, 35 μM CaM, ~ 20 μM dRF and 5 mM fresh semicarbazide in pH 7.6 buffer (40 mM Bis-Tris propane, 400 mM NaCl, 2 mM l-Arg, 20 μM H4B, 1 mM Ca2+ and 10 % glycerol). The sample was well degassed by Ar/CO (3:1).