Table 1.

Rates of the FMN–heme IET of NOS holoenzymes determined by CO photolysis ^a

	k (s-1)	Ref.
CaM-bound eNOS	4.3 ± 1.0 b	This work
CaM-bound nNOS	36 ± 4	[29]
iNOS	35 ± 4	[27]

^aThe IET kinetics were determined under the same conditions at room temperature; buffer: 40 mM Bis-Tris propane, 400 mM NaCl, 2 mM l-Arg, 20 μM H₄B, 1 mM Ca²⁺ and 10 % glycerol, pH 7.6.

^bThe absolute value of the rate constant could be uncertain by ± 100 % (due to small S/N). The error value of ± 1.0 s^-1 given in the Table reflects only the standard error in the computer fit to the data.