Table 6.

Hepatic P450-dependent Dealkylase Activities in Microsomal Fractions of Cyp1a2−/−;Hfe−/−;Urod+/− Mice After Treatment Regimens Consisting Variously of High Iron Diet, Iron-dextran and PCB injections and δ-Aminolevulinic acid-containing Drinking Water

CYP SUBSTRATE	mouse	Hepatic P450 Activities (nmol/mg microsomal protein/min)
		Treatment 2	Treatment 3	Treatment 4	Treatment 5
		Fe-diet	Fe-diet ala/fedex	Fe-diet pcb/ala	Fe-diet pcb/ala/fedex
7-methoxyresorufin
	#1	0.025	0.020	0.828	0.263
	#2	0.029	0.020	1.018	1.099
	#3	0.015	-	1.142	0.861
	#4	0.020	0.026	1.518	1.147
	#5		0.014		1.216
	#6		0.027		1.133
7-ethoxyresorufin
	#1	0.100	0.056	2.259	1.119
	#2	0.144	0.078	3.269	3.429
	#3	0.063	0.052	3.594	2.609
	#4	0.113	0.106	5.060	4.051
	#5		0.051		4.106
	#6		0.085		3.858
7-ethoxy-4-trifluoro methylcoumarin
	#1	0.573	0.274	3.692	1.678
	#2	0.605	0.327	3.970	4.221
	#3	0.481	0.386	4.202	3.987
	#4	0.455	0.389	5.994	4.795
	#5		0.354		4.209
	#6		0.457		4.386
7-pentoxyresorufin
	#1	0.049	0.043	0.051	0.065
	#2	0.053	0.039	0.050	0.065
	#3	0.039	0.046	0.048	0.044
	#4	0.046	0.025	0.060	0.048
	#5		0.041		0.053
	#6		0.040		0.051
7-benzyloxy quinoline
	#1	0.719	0.462	0.418	0.229
	#2	0.701	0.413	0.605	0.608
	#3	0.520	0.430	0.560	0.369
	#4	0.484	0.446	0.956	0.609
	#5		0.322		0.624
	#6		0.529		0.699

Mice fed a high iron diet (fe-diet, 2 mg Fe/g) after weaning > 14 weeks and were sacrificed 4 weeks after receiving any of additional treatments indicated. “Fedex” was a single injection of aqueous iron dextran (10 mg, ip), “ala” was δ-aminolevulinic acid (2 mg/ml) provided continuously in the drinking water and “pcb” was a single injection of Aroclor 1254, (4 mg, ip in corn oil). Values for animals are arranged in the same order as the male Cyp1a2−/− Hfe−/− Urod+/− group in Table 2.