Table 3. NCBI-nr reference genes with significantly different abundances in metatranscriptomes generated from vacuum filtered vs ESP-filtered and preserved replicate samplesa.
| NCBI-nr reference gene | Vacuumb | ESP b | P-value |
|---|---|---|---|
| AAM48736 antenna pigment protein, alpha chain (uncultured marine proteobacterium) | ND | 0.22% | 6.1 × 10−08 |
| ZP_01224258 hypothetical protein (gamma proteobacterium HTCC2207) | 0.25% | 0.67% | 4.1 × 10−05 |
| ZP_01447883 branched-chain amino acid ABC transporter, periplasmic substrate-binding protein (Rhodobacterales sp. HTCC2255) | 0.54% | 0.17% | 5.4 × 10−05 |
| ZP_03559919 50S ribosomal subunit protein L3 (Glaciecola sp. HTCC2999) | 0.12% | ND | 8.5 × 10−03 |
| ZP_03559922 50S ribosomal protein L2 (Glaciecola sp. HTCC2999) | 0.10% | ND | 0.041 |
| ZP_01447418 glutamate synthase large subunit (Rhodobacterales sp. HTCC2255) | 0.14% | 0.01% | 0.043 |
Abbreviations: ESP, Environmental Sample Processor; FDR, false discovery rate; ND, not determined; nr, non-redundant.
a
Reference genes with FDR-corrected P-value >0.05.
b
The percentage of unique, non-rRNA reads with significant NCBI-nr database hits mapping to each reference gene in vacuum-filtered and the ESP-processed and preserved samples.