Figure 1. Wnt signaling is active at the anterior border of Cdx2+ endoderm between E7.5–E8.5.
(A) Wholemount immunofluorescence image of E7.5 BAT-Gal embryo stained for β-gal. Boxed area represents embryonic region imaged in higher detail in B–D. (B–D) Wholemount confocal immunofluorescence images of E7.5 BAT-Gal embryo stained for β-gal (B, green in D), Cdx2 (C, red in D), and Foxa2 (blue in D). (E) Wholemount immunofluorescence image of E8.25 BAT-Gal embryo stained for β-gal. Boxed area represents embryonic region imaged in higher detail in F–H. (F–H) Wholemount confocal immunofluorescence images of E8.5 BAT-Gal embryo stained for β-gal (F, green in H), Cdx2 (G, red in H), and Foxa2 (blue in H). (I) Wholemount immunofluorescence image of E8.75 BAT-Gal embryo stained for β-gal. Scale bar equals 500 µm in A, E, and I and 50 µm in D and H.