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. 1999 Jan;119(1):31–40. doi: 10.1104/pp.119.1.31

Figure 7.

Figure 7

Changes in the level of the Cdc2Os3 protein in response to cell-cycle blockers. a, Specific cross-reactions of the GST-Cdc2Os2 and GST-Cdc2Os3 fusion proteins with antibodies. One microgram of each GST-fusion protein was subjected to immunoblotting with antibodies against Cdc2Os2 or Cdc2Os3. Lanes 1, GST-Cdc2Os1; lanes 2, GST-Cdc2Os2; and lanes 3, GST-Cdc2Os3. b, Immunological detection of Cdc2Os2 (p40) and Cdc2Os3 (p36) in suspension-cultured rice cells that had been treated with cell-cycle blockers. Total protein was extracted from cultured cells after no treatment (NT) and after treatment with colchicine (CO) or hydroxyurea (HU). Twenty micrograms of each sample was subjected to immunoblotting with Cdc2Os2- or Cdc2Os3-specific antibodies. c, Histone H1-kinase activities in the immunoprecipitates obtained with the antibodies. Total protein was extracted from cultured cells as described above and used for immunoprecipitation with Cdc2Os2- or Cdc2Os3-specific antibodies. Immunoprecipitates were subjected to the histone H1-kinase assay, and phosphorylated histone H1 was detected.