Fig. 6.

Decreased cholesterol absorption in ApoE^−/−Dgat1^−/− mice. Mice were gavaged with 200 μl corn oil containing 2 μCi ³H-cholesterol and 0.2 mg cholesterol. 4 h later, absorption in (A) liver and (B) plasma and (D) intestinal uptake of ³H-cholesterol was determined by liquid scintillation counting. ³H-Cholesterol in HDL was assayed after precipitation of ApoB-containing lipoproteins using Quantolip HDL (Technoclone GmbH). (C) Plasma samples from each genotype (n = 6) were pooled and 200 μl were subjected to fast protein liquid chromatography using a Superose 6 column. ³H-Cholesterol in each fraction was determined by liquid scintillation counting. (E) Fractional cholesterol absorption was determined using the fecal dual-isotope method. Cholesterol biosynthesis in (F) liver and (G) jejunum was determined 1 h after ¹⁴ C-acetate administration. Data represent mean values (n = 4–5) ± SEM. *p < 0.05; **p ≤ 0.01; ***p ≤ 0.001.