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. 2011 Nov 7;108(47):19007–19012. doi: 10.1073/pnas.1109066108

Fig. 1.

Fig. 1.

Vβ2 NKT TCR affinity measurements. Sensorgrams illustrate binding of graded concentrations of Vα14-Vβ2+ (column 1; 1.3–0.016 μM for α-GalCer and 9.3–0.002 μM for other ligands), Vα14-Vβ7+ (column 2; 56–0.015 μM), and Vα14-Vβ8.2+ (column 3; 2.1–0.004 μM for α-GalCer and 35–0.009 μM for other ligands) soluble NKT TCRs to CD1d-α-GalCer, CD1d-α-GlcCer, CD1d-3′,4″-deoxy α-GalCer, CD1d-4′,4″-deoxy α-GalCer, and CD1d–OCH after subtraction from a control (CD1d-endogenous) flow cell. Saturation plots (column 4) show equilibrium binding. The dissociation constant (KD) derived by equilibrium analysis, association rate (ka; M−1⋅s−1), and half-life (t1/2) is shown for each interaction. The data shown is from one experiment and is representative of three separate experiments for CD1d-α-GalCer and CD1d-α-GlcCer and two separate experiments for the other ligands.