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Impact of CD1d mutants on NKT TCR binding. Mouse CD1d-α-GalCer tetramers (WT or mutants) were assessed for their ability to bind Vβ2+, Vβ7+, and Vβ8.1/8.2+-enriched thymic NKT cells. CD3+NK1.1+ Vβ+ cells were examined for mean fluorescence intensity (MFI) of mCD1d/α-GalCer tetramer. Data are shown as a percentage of WT and are from three independent experiments with each experiment represented by a symbol.
