Fig. 2.

Cartoons conceptualizing possible DnaK–DnaJ interactions. (A) DnaK (Hsp70) is on top, with NBD in red and SBD in blue. The LID domain is not shown. A DnaJ dimer is at the bottom, with the J domains in yellow, the GF regions in magenta, the SBDs in cyan, and the dimerization helices in brown. Ellipses represent constructs used in this study. (B) Bivalent interaction of a DnaJ monomer with DnaK. The area in the ellipse corresponds to the complex between DnaJ(1–108) and DnaK. (C) The trans-complex between DnaK and DnaJ with substrate peptide (NR). The area in the ellipse corresponds to the complex between DnaJ(1–108) and DnaK with NR. (D) The cis-triple complex between DnaK, DnaJ, and substrate protein. The area in the ellipse corresponds to the complex between DnaJ(1–70) and DnaK(1–605) with NR determined in this work (Fig. 4). (E) As in D, but in context of a hypothetical oligomeric complex involving DnaJ dimers.