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Reduction in signal height of the DnaJ(1–70) NH TROSY heteronuclear single quantum correlation cross-peaks due to the presence of spin-labeled DnaK. (Top) DnaK(1–605)V210C-MTSL and NR (DnaJ∶DnaK ratio: blue, 1∶0; green, 1∶0.5; red 1∶1.2). (Middle) As above, but with DnaK(1–605)D326C-MTSL and NR (DnaJ∶DnaK ratio: blue, 1∶0; red 1∶1.2). (Bottom) With DnaK(1–605)K421C-MTSL and NR (DnaJ∶DnaK ratio: blue, 1∶0; red 1∶1.2)
