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Phosphorylation of ERK1/2 in MO59J spheroids after treatment with 5 Gy irradiation or gefitinib, alone or in combination. ERK1/2 phosphorylation was determined by phospho-ERK immunoblotting using anti-phospho ERK1/2 antibody, and anti-ERK1/2 antibodies against total ERK1/2. Lane 1 - control; lane 2- rhEGF (25 ng/mL); lane 3 - 5 Gy irradiation, lane 4 - gefitinib (50 μM) and lane 5 - 5 Gy irradiation plus gefitinib (50 μM).
