Figure 3.

Proinflammatory cytokine treatment increased the lung weight/body weight ratio and induced alveolar exudation of EV71-infected, but not HSV-1-infected mice. Seven-day-old ICR mice (n = 12) as treated as described in Figure 2. Their lung weight/body weight ratio was determined (A and B), and lung tissues were collected for gross (C) and histopathological examination (H & E stain) (D) at day 1 after the cytokine treatment. BSA: mice received BSA only; ×2: mice received 2 doses of cytokines within 48 h; ×3: mice received 3 doses of cytokines within 48 h. Note interlobular edema (arrow) (C) and eosinophilic, proteinaceous exudation in alveolar spaces with moderate to severe emphysema in EV71-infected mice with cytokine treatment. Bars: 1.0 mm (left panel), 200 μm (right panel). (D). Data represent means ± S.E.M.