Table 1.
Primers | positions of 5'end @ | Sequences of primers (5'-3') | |
---|---|---|---|
3' adaptor outer primer | TACCGTCGTTCCACTAGTGATTT | ||
3' adaptor inner primer | CGCGGATCCTCCACTAGTGATTTCACTATAGG | ||
5' adaptor outer primer | CATGGCTACATGCTGACAGCCTA | ||
5' adaptor inner primer | CGCGGATCCACAGCCTACTGATGATCAGTCGATG | ||
M13F | GTTTTCCCAGTCACGAC | ||
M13R | CAGGAAACAGCTATGAC | ||
F1 | UL87 AS 5' RACE primers |
129553 | GAGAACCCGACCCGTAAA |
F2 | 129806 | GTCGGAGACGGGAGAAG AGG | |
F3 | 130459 | GTTCAAAGCGGCTACGGCCAT | |
F4 | 130029 | AACGCTTTCAACACCAACCGCG | |
R1 | UL87 AS 3' RACE primers |
129829 | CTCCTCTTCTCCCGTCTCC |
R2 | 130049 | GCGGTTGGTGTTGAAAGC |