Table 1.

NMR analysis of the UXS assay product. Chemical shifts (¹H and ³¹P NMR) are expressed as ppm. Approximately 300 μg of HPLC-purified reaction product was lyophilised twice and taken up in 0.6 ml D₂O. Spectra were recorded at 300 K at 600.18 MHz for ¹H and at 242.96 MHz for ³¹P with a Bruker AV III 600 spectrometer. Data acquisition and processing were performed with the standard Bruker software. ¹H spectra were referenced internally to sodium 2,2-dimethyl-2-silapentane-5-sulphonate and ³¹P spectra to phosphoric acid (δ = 0).

	H1	H2	H3	H4	H5, 5′	H6	α	β
Xylose	5.59	3.55	3.69	3.61	3.71
Ribose	6.01	4.41	4.44	4.27	4.24
Uracil					6.02	7.99
Pyrophosphate							−11.28 (19.5 Hz)	−12.98 (21.4 Hz)