Fig. 3.

Knockdown of RNase H1, but neither FEN1 nor DNA2 inhibits mtDNA replication. Cells were transiently treated with 2′,3′-dideoxycytidine and transfected with scramble (Sc) dsRNA, RNase H1-specific (RH1) dsRNA, FEN1-specific (FEN1) dsRNA, DNA2-specific (DNA2) dsRNA and both FEN1 dsRNA and DNA2 dsRNA (F + D) as in the diagram of Fig. 1A. (A) Western blot analysis of RNase H1, FEN1 and DNA2 levels. Below each panel Tubulin is shown as loading control. (B) The relative copy number of mtDNA analysed with real-time quantitative qPCR. The interpretation of the graph is as in Fig. 1C. Data represent the mean of 3 independent transfection experiments ± S.E.M. * (p < 0.05).