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Comparison of geometries and Gibbs energy profiles of the pro-RP/pro-RP/G8/A38H+ (green) and pro-RP/pro-RP/G8/A38 (white and cyan) reaction mechanisms, i.e., in presence of protonated A38H+ and canonical A38 form. All energies are relative to the R’ state that represents the reactant with the dominant protonation states of G8 and A38 at pH ~7, i.e. both nucleobases in canonical forms.
