Method 2. Seed regions in the optic tract (red) in the axial (a) and coronal planes (b). Large regions of interest (ROIs) including voxels above and below the calcarine fissure (red) lateral to the midline in the axial (a) and sagittal planes (c), used both as seed region for the identification of the LGN region and as an endpoint for the final identification of the optic radiation. Seed regions in the LGN region (pink) in axial (d) and coronal planes (e, and f), with the voxel (green) closest to the LGN where the optic tract and optic radiation overlap. Exclusion masks (green) in the midline encompassing both the thalamus and corpus callosum (h), in the thalamus in a coronal plane (i), at the level of the trigone (j) and all three shown in the axial plane (g).