Figure 4.

Histograms of the chain-length distribution of starch-like polysaccharides obtained through gel electrophoresis of fluorescent glucans after isoamylase-mediated enzymatic debranching. Both chain-length distributions of debranched high-molecular-mass (amylopectin-like; A) and debranched low-molecular-mass (amylose-like; B) fractions are displayed as percentages of chains of DP ranging from 3 to 20. Separation of both fractions was achieved through TSK-HW-75 gel-permeation chromatography of granular polysaccharides (Fig. 2) produced in the sta7 glycogen-synthesizing mutants.