Figure 6.

Histograms of chain-length distributions obtained through gel electrophoresis of fluorescent glucans after isoamylase-mediated enzymatic debranching of phytoglycogens produced by double or triple C. reinhardtii mutants. Chain-length distributions of debranched phytoglycogens from sta2 sta7 (A), sta3 sta7 (B), and sta2 sta3 sta7 (C) mutant strains are displayed as percentages of chains of DP between 2 and 16. Phytoglycogen purification was achieved by taking the high-mass fraction after TSK-HW-50 gel-permeation chromatography, as displayed in Figure 5.