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Expression of MITF and HERV-K mRNAs in normal and transformed melanocytes. Semiquantitative RT-PCR was performed with RNA purified from normal human melanocytes (NHM/FBS-free) and melanoma lines, G361, SK-MEL-28, and MeWo. NHM cultured with 1% FBS for 9 hours (NHM/1% FBS) was also analyzed. MITF-M, total MITF, and HERV-K mRNAs (gag, env, and rec), and 18S rRNA were amplified with primers shown in Figure 6.
