Table 1.
Genes and primers used to amplify targets for plasmid standards (PCR) and measure transcript abundance (qRT-PCR) in T. pseudonana.
| Target gene/protein ID/Accession number | Reaction type | Primer direction | Primer sequence (5′–3′) |
|---|---|---|---|
| Fe reductase (chr 3)/3129/XP_002288167 | PCR | Forward | TCAACGTTTTCTTCTGGAGTCA |
| Reverse | ATTCTCCGCAGCACATTTTT | ||
| qRT-PCR | Forward | TCGTGGGCATGTATCTTCATG | |
| Reverse | GCCTCCGTAGCAGACTATTGATG | ||
| Probea | TCCTGGTGCTCGTCCCTTCATCCTC | ||
| Fe reductase (chr 19)/11375/XP_002297175 | PCR | Forward | TGGTAGCACATCCTTTCACGGTCA |
| Reverse | ATGGAGAGAAACGTTGCAAACGGC | ||
| qRT-PCR | Forwardc | GCCGTACTTGACAATGCTGA | |
| Reversec | ACCAGTGAAATGCACGATGA | ||
| Probea | AAGTCTCCTCTCACGAAATCAGTGGAGC | ||
| Multicopper oxidase/5574/XP_002290775 | PCR | Forward | TGGGAGTTTGGTACATGCAA |
| Reverse | GCGACACATCCAGAAGGTTT | ||
| qRT-PCR | Forward | CGGTGGTGGCTGCTGATT | |
| Reverse | TCTCCGACGTGTACCACAACTT | ||
| Probea | AGTTGAAGCGTTTACTGTAGAC | ||
| Cu transporter (chr 7)/35496/XP_002295670 | PCR | Forward | GTCGTTTGGGCATTGCTACT |
| Reverse | CAATCACCTTCTGACGAGCA | ||
| qRT-PCR | Forward | GCCTGCCCTTGTGCATTG | |
| Reverse | TGCTCCTATTCCGGTTCCAA | ||
| Probea | ATTGGCCACGCCTACTGCCGTCAT | ||
| Cu transporter (chr 16)/264357/XP_002296688 | PCR | Forward | CCAACGGATGGAGTGCTAAT |
| Reverse | AGGGACACGCCACTACAATC | ||
| qRT-PCR | Forward | TCCAAGCTCAGGCTGATCGT | |
| Reverse | TGTAGCGGCAGCAACTAGCA | ||
| Probea | TTGCATCCATCTTTGCTCCGATCGTC | ||
| Flavodoxin/28635/XP_002291468 | PCR | Forward | AAGTGGGCGTCTTCTTCGGTACTT |
| Reverse | TCTCCACGCTGAGCCTTTGATTCT | ||
| qRT-PCR | Forward | TGCGAAGTATGATGCTTTAGTGGTA | |
| Reverse | CCAGCCCGTTCCACTACGT | ||
| Probea | CCAACATGGAACACTGGTGCTGACACTG | ||
| Ferredoxin/YP_874492 | PCR | Forward | GTCTGATCTTCCAAAGATTCAAATCGGG |
| Reverse | CCAGCACCCATTTGATCATCATC | ||
| qRT-PCR | Forward | AGATGCAGCAGAAGAAGCAGGA | |
| Reverse | ACCATCTGATACTTTACCAGCACA | ||
| Probea | CTTGTCGTGCTGGTGCTTGTTCTACA | ||
| Actin/25772/XP_002294917 | PCR | Forward | ACGTGACCTCACGGACTACC |
| Reverse | CAAAGCCGTAATCTCCTTCG | ||
| qRT-PCR | Forward | GTGGACTTTGAGGAGGAGATGAA | |
| Reverse | CAGGGAGCTCGAAGGACTTCT | ||
| Probeb | GGCTGCGGAGTCGTCGGCTCT |
aProbe labeled with 5′-FAM and 3′-BHQ.
bProbe labeled with 5′-HEX and 3′-BHQ.
cPrimer pair used in Kustka et al. (2007).