Figure 1.

Modelling the effects of transient and sustained GnRH on cytoplasmic Ca²⁺ concentration and NFAT translocation. (a) Model predictions for effects of GnRH (10⁻⁷ M) on [Ca²⁺]_i in cells stimulated either continuously (horizontal arrow) or for 5 min (grey rectangle) from t = 0 (solid line, GnRH pulse; dashed line, GnRH constant). (b) Experimental data obtained under these conditions using HeLa cells transduced with GnRHR and loaded with fura 2 for Ca²⁺ measurement. (c) Model predictions for translocation of NFAT from the cytoplasm to the nucleus (N:C NFAT ratio) in cells stimulated for 5 min or continuously with 10⁻⁷ M GnRH and (d) experimental data under these conditions using HeLa cells transduced to express GnRHR and NFAT1c-EFP. See [23] for detailed methods.