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Endothelial cell tube formation was enhanced by plasma treatment. PAEC were plasma-treated (4.2 J cm–2) and polymerized in a collagen gel. (a) Phase contrast images of tube formation at 24 h. Samples were plasma-treated in the presence of an FGF-2 neutralizing antibody or the extracellular ROS scavenger SP. FGF-2 (10 ng ml–1) was the positive control. (b) Tube length was quantified using ImageJ. †p < 0.001 compared with control, +p < 0.0001 compared with plasma.
