Structure of RNS1 and
RNS2 antisense constructs p1448, p1449, p1525, and
p1527. A, General structure of the T-DNA region of the antisense
RNS transformation vectors. B, RNS cDNA
fragments fused in the antisense orientation between the doubly
enhanced cauliflower mosaic virus 35S promoter and the
nos 3′ end. Sequences flanking the 35S-antisense
RNS-nos cassette are derived from the
plasmid pBI121, as described previously (Jefferson, 1987). Numbers
refer to nucleotide positions in the cDNA sequences. Triangles indicate
the right and left borders of the T-DNA.