Figure 6.

Decreased RNS1 activity and protein in RNS1 antisense lines. T₃ or T₄ seedlings of antisense RNS1 lines were germinated on AGM medium, transferred 2 d after germination to media rich (+) or deficient (−) in Pi, and grown for an additional 7 d. Protein extracts were prepared from all kanamycin-resistant seedlings and each sample was resolved on RNase activity gels (A, 50 μg per lane) or SDS-PAGE gels for immunoblots (B, 100 μg per lane). wt Col, Columbia wild type; vector control, transgenic line containing pBI121 vector; weak antisense, transgenic line containing construction p1448 but with a near-normal amount of RNS1 activity; RNS1, RNS1 protein produced in yeast. The bands of RNS1 activity are indicated. Positions of molecular mass standards (in kD) are shown to the left of the gels.