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Growth-phase-dependent expression of tobacco CycD cyclins. Total RNA isolated from stationary-phase BY-2 cells (7 d after previous subculture) and exponentially growing cells (3 d after subculture) was blotted and hybridized to the indicated cDNA probes. The membrane was stained in methylene blue prior to hybridization to visualize the principal rRNA bands to control for loading (Methods). Note that CycD3;2 is approximately 10- to 20-fold more abundant than the other two cyclins.
