Figure 7.

Expression of CycD3;2 in cells treated with cell-cycle inhibitors. Cells were arrested in the S phase with aphidicolin or in mitosis with oryzalin or propyzamide (Methods) and harvested for RNA analysis. Cell-cycle arrest was confirmed by determining the mitotic index (MI) and the level of histone H4 transcripts. Samples from stationary and exponentially dividing (3 d after subculture) cells are included for a comparison. The loading control was by methylene blue staining of the membrane.