Figure 2. Effects of HDL from healthy subjects and patients with sCAD and ACS on endothelial Akt activation, eNOS-activating/inhibiting phosphorylation, and endothelial superoxide production.

(A) Effects of HDL (50 μg/ml) on phosphorylation of Akt at Ser473, (B) Akt-dependent activating phosphorylation of eNOS at Ser1177, and (C) inhibitory phosphorylation of eNOS at Thr495 in HAECs, as detected by Western blot analysis (n = 10–12 per group). (D) Effects of HDL on endothelial superoxide production (n = 25 per group; data points for all study participants are shown) and (E) NAD(P)H oxidase activity, as measured by ESR spectroscopy (n = 8–10 per group). (F) Representative ESR spectra of NAD(P)H oxidase activity in HAECs treated with buffer (basal) and with HDL from healthy subjects or patients with sCAD or ACS.