Skip to main content
. 2011 Jun 13;121(7):2625–2640. doi: 10.1172/JCI43068

Figure 3. VLDLR expression is dependent on HIF-1α in HL-1 cells.

Figure 3

(A) Representative immunoblots of HIF-1α, VLDLR, and β-actin in HL-1 cells transfected with control siRNA or Hif1a #1 siRNA and incubated in normoxia or hypoxia for 8 hours. (B) Real-time quantitative RT-PCR analysis of Vldlr mRNA expression (n = 6) (left) and representative immunoblots (right) of HIF-1α, VLDLR, and β-actin in normoxic HL-1 cells incubated with DMSO or the HIF-1α stabilizer DMOG (1 mmol/l) for 8 hours. (C) The total area of Oil Red O–stained lipid droplets in normoxic HL-1 cells transfected with control siRNA and incubated with DMSO (control), DMOG (1 mmol/l), or Vldlr #1 siRNA plus DMOG (1 mmol/l) for 8 hours (n = 9). Results are shown as mean ± SEM.