Skip to main content
. 2011 Jun 1;121(7):2723–2735. doi: 10.1172/JCI44745

Figure 4. Importance of the IL-6/JAK2/Stat3 pathway in tumor growth.

Figure 4

(A) Representative immunofluorescence staining patterns for CD44, CD24, and pStat3 in ER–PR–HER2+ inflammatory breast carcinoma (IDC31). Scale bar: 10 microns. (B) Representative immunofluorescence staining patterns for CD44, CD24, and pStat3 in SUM159PT and IDC31 xenografts. Scale bars: 10 microns. (C) Box plots showing percentage of pStat3+ cells in SUM159PT and IDC31 mouse xenograft-derived (IDC31-X) xenografts by immunofluorescence and immunohistochemistry, respectively (counting 2–6 fields per sample). (D) Box plots showing xenograft tumor weights 34, 28, 28, 40, or 70 days after injecting SUM159PT, MDA-MB-231, MDA-MB-468, Hs 578T, or IDC31-X cells, respectively, into 2 fat pads of n mice. Mice were administered daily NVP-BSK805 (2 mg/mouse) or vehicle only (control) for 14, 16, 16, 24, or 24 days, respectively (after tumors reached palpable size), beginning 21, 13, 13, 17, or 47 days after injection, respectively. (E) H&E-stained Hs 578T and IDC31-X xenografts. (F) Box plots showing the percentage of area with cells in Hs 578T and IDC31-X xenografts calculated from whole tumor sections with H&E staining. (G) Kaplan-Meier curves of SUM159PT xenografts expressing STAT3 shRNAs (shSTAT3 #1 and #2) in n mice. (H) Immunoblots with cells used for xenografts in G. Tubulin was used as a loading control. (I) pStat3 immunohistochemistry staining for xenografts in G. Scale bars: 50 microns. Triangles in C, D, and F mark averages. *P < 0.05; **P < 0.01; ***P < 0.001, t test (C, D, and F). ***P < 0.001, log-rank test (comparing each STAT3 shRNA group to the shGFP control group) (G).