Skip to main content
. 2011 Jun 13;121(7):2736–2749. doi: 10.1172/JCI45444

Figure 3. KLF4 deficiency enhances macrophage M1 polarization.

Figure 3

(A) Enhanced expression of M1 marker genes in KLF4-deficient macrophages stimulated by LPS for 16 hours. n = 3 in each group. (B) Attenuated expression of M1 marker genes in RAW264.7 cells with overexpression of KLF4. n = 3 in each group. (C) Protein levels of Cox-2 and iNOS in PMs (left) and RAW264.7 cells (right). (D) KLF4-deficient macrophages generate more NO after LPS stimulation. n = 3 in each group. (E) Inhibition of Cox-2 promoter by KLF4. Transient transfection experiments were performed in RAW264.7 cells. n = 3. (F) Enhanced acetylation of histone H3 (AcH3) in LPS-treated KLF4-deficient macrophages demonstrated by ChIP. (G and H) KLF4 regulates LPS-induced recruitment of p300/PCAF as shown by deficiency (G) and overexpression (H) experiments. *P < 0.05, **P < 0.01, Student’s t test with Bonferroni correction.