Figure 8. Effect of PKCδ inhibition on cisplatin-induced apoptosis in multiple cancer cell lines.

(A) Effect of PKCδ inhibitor δV1-1 on cisplatin-induced apoptosis in cancer cells. Indicated cell lines were pretreated with 2 μM Tat or δV1-1 peptide for 1 hour in reduced serum medium before treating with 25 μM cisplatin for 24 hours, and apoptosis was monitored as described in Methods. Mean ± SD, n = 3. *P < 0.05 versus cisplatin plus Tat group. (B) Effect of genetic inhibition of PKCδ on cisplatin-induced apoptosis. Indicated cell lines were transfected with either empty vector, PKCδ-KD, scrambled siRNA, or PKCδ-siRNA, and 48 hours after transfection, the cells were treated with 20 μM cisplatin for 24 hours, and apoptosis was estimated. Mean ± SD, n = 3. *P < 0.05 versus vector group; ^#P < 0.05 versus scrambled siRNA group.