Figure 9. Rottlerin ameliorates cisplatin-induced kidney injury without blocking the therapeutic effects in human ovarian tumor xenografts.

Tumor xenografts were established in athymic nude mice by inoculation of A2780 ovarian cancer cells. After the tumors had grown to approximately 200 mm³, the animals were then randomly divided into 3 groups (11 mice/group), which were treated weekly with saline, 10 mg/kg cisplatin, or 10 mg/kg cisplatin plus 10 mg/kg rottlerin. (A) Tumor volume during treatment. Tumors were measured each week to determine tumor volume. (B) Representative mice and dissected tumors. (C) BUN values during the treatment. (D) Serum creatinine levels during the treatment. (E) Representative renal histology and TUNEL staining of tissues collected after 4 weeks of treatment. Original magnification, ×200. Asterisks in E indicate lysed tubules, and arrows indicate TUNEL-positive nuclei. (F) Animal death and survival during the treatment. Mean ± SD. *P < 0.05 versus untreated saline control group; ^#P < 0.05 versus cisplatin-only group.