FIGURE 4.
S6K1 phosphorylation on Thr389 (A), 4EBP1 phosphorylation on Thr70 (B), inactive 4EBP1·eIF4E complex abundance (C), and active eIF4E·eIF4G complex abundance (D) in the longissimus dorsi muscle of intermittently bolus-fed, continuously fed, and feed-deprived neonatal pigs. All results are corrected for total protein. Values are means ± SEM, n = 5–7. ANOVA showed an effect of treatment on S6K1 and 4EBP1 phosphorylation and 4EBP1·eIF4E and eIF4E·eIF4G abundance, P < 0.05. Means without a common letter differ, P < 0.05. eIF, eukaryotic initiation factor; S6K1, ribosomal protein S6 kinase 1.