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. 1999 Jan;119(1):353–362. doi: 10.1104/pp.119.1.353

Figure 5.

Figure 5

Heterologous expression of the recombinant AtCBR protein in insect cells. A, SDS-PAGE was performed using a 16% polyacrylamide slab gel, and proteins were visualized by staining with Coomassie brilliant blue R-250. Lane 1, Molecular mass marker proteins; lane 2, 100,000g precipitate of mock-infected Sf21 cells; lane 3, 100,000g precipitate of the Sf21 cells infected with the recombinant virus containing the full-length AtCBR cDNA; lane 4, 100,000g supernatant of the AtCBR-expressing Sf21 cells; lane 5, solubilized fractions of 100,000g precipitate of the AtCBR-expressing Sf21 cells; lane 6, the purified recombinant AtCBR. B, Absolute absorption spectrum of the purified recombinant AtCBR in its oxidized form (solid line) and reduced by the addition of 100 μm NADH (dashed line).