Table 2.
Antigen | Company and Identification No. | Host, Immunogen | Fixation Method | Working Dilution |
---|---|---|---|---|
Cav1.2 | Alomone (Jerusalem, Isreal), ACC-003, lot AN-18 | Rabbit polyclonal, seq 848–865 aa, intracellular loop between domain II–III |
4% PFA | 1:75 |
Cav1.3 | NeuroMab (Davis, CA), 75-080 | Mouse monoclonal, clone N38/8, fusion protein 2025–2161 aa, C- terminus |
Methanol | 1:100 |
Cav2.1 | Alomone (Jerusalem, Isreal), ACC-001, lot AN-09 | Rabbit polyclonal, seq 865–881 aa, intracellular loop between domain loop II–III |
Methanol | 1:1400 |
Cav2.1 | Sigma-Aldrich (St. Louis, MO), C1353 | Rabbit polyclonal, seq 865–881 aa, intracellular loop between domain II–III |
Methanol | 1:25 |
Cav2.2 | Alomone (Jerusalem, Israel), ACC-002, lot AN-01 and −21 | Rabbit polyclonal, seq 851–867 aa, intracellular loop between domain II–III |
Methanol | 1:400, 1:100 |
Cav2.3 | Alomone (Jerusalem, Israel), ACC-006; lot AN-03 | Rabbit polyclonal, seq 982-907 aa, intracellular loop between domain II–III |
4% PFA | 1:50 |
Cav3.1 | Alomone (Jerusalem, Israel), ACC-021; lot AN-05 | Rabbit polyclonal, seq 1–22 aa, N-terminus | 4% PFA | 1:50 |
Cav3.3 | Alomone (Jerusalem, Israel), ACC-009, lot AN-02 | Rabbit polyclonal, seq 1053–1067 aa, intracellular loop between domain II–III |
Methanol | 1:50 |
MAP-2 | Millipore (Billerica, MA), AB5622, lot NG1723932 | Rabbit polyclonal, microtubule-associated protein | Methanol | 1:100 |
MBP | Sternberger Monoclonals Inc. (Lutherville, MD), lot 1 | Mouse monoclonal, clone SMI-99 | 4% PFA | 1:1000 |
β-tubulin | Covance (Princeton, NJ), MMS-435P | Mouse monoclonal, neuronal class III β-tubulin | Methanol / 4% PFA | 1:200 |
β-tubulin | Covance (Princeton, NJ), PRB-435P | Rabbit polyclonal, neuronal class III β-tubulin | Methanol / 4% PFA | 1:200 |