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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Hear Res. 2011 Jan 31;278(1-2):52–68. doi: 10.1016/j.heares.2011.01.016

Table 2.

Antibody information for immunocytochemistry.

Antigen Company and Identification No. Host, Immunogen Fixation Method Working
Dilution
Cav1.2 Alomone (Jerusalem, Isreal), ACC-003, lot AN-18 Rabbit polyclonal, seq 848–865 aa, intracellular loop between
domain II–III
4% PFA 1:75
Cav1.3 NeuroMab (Davis, CA), 75-080 Mouse monoclonal, clone N38/8, fusion protein 2025–2161 aa, C-
terminus
Methanol 1:100
Cav2.1 Alomone (Jerusalem, Isreal), ACC-001, lot AN-09 Rabbit polyclonal, seq 865–881 aa, intracellular loop between
domain loop II–III
Methanol 1:1400
Cav2.1 Sigma-Aldrich (St. Louis, MO), C1353 Rabbit polyclonal, seq 865–881 aa, intracellular loop between
domain II–III
Methanol 1:25
Cav2.2 Alomone (Jerusalem, Israel), ACC-002, lot AN-01 and −21 Rabbit polyclonal, seq 851–867 aa, intracellular loop between
domain II–III
Methanol 1:400, 1:100
Cav2.3 Alomone (Jerusalem, Israel), ACC-006; lot AN-03 Rabbit polyclonal, seq 982-907 aa, intracellular loop between
domain II–III
4% PFA 1:50
Cav3.1 Alomone (Jerusalem, Israel), ACC-021; lot AN-05 Rabbit polyclonal, seq 1–22 aa, N-terminus 4% PFA 1:50
Cav3.3 Alomone (Jerusalem, Israel), ACC-009, lot AN-02 Rabbit polyclonal, seq 1053–1067 aa, intracellular loop between
domain II–III
Methanol 1:50
MAP-2 Millipore (Billerica, MA), AB5622, lot NG1723932 Rabbit polyclonal, microtubule-associated protein Methanol 1:100
MBP Sternberger Monoclonals Inc. (Lutherville, MD), lot 1 Mouse monoclonal, clone SMI-99 4% PFA 1:1000
β-tubulin Covance (Princeton, NJ), MMS-435P Mouse monoclonal, neuronal class III β-tubulin Methanol / 4% PFA 1:200
β-tubulin Covance (Princeton, NJ), PRB-435P Rabbit polyclonal, neuronal class III β-tubulin Methanol / 4% PFA 1:200